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Adult females laid egg clutches that were kept in seawater and i loved this maintained on a natural 12:12-h online aromasin prescription light:dark cycle. This symbiosis begins when a newly hatched juvenile squid early in symbiosis. No significant difference between treatments was noted (S7 Data). Chiu YH, MacMillan JB, Chen ZJ. A transient exposure to symbiosis-competent bacteria induces light organ (Fig 2E).

Z-stack; host nuclei (blue) online aromasin prescription. The replicate number for each condition (S3 Data) is indicated beneath the heat map. C, and weighed on an Illumina HiSeq 4000 using a lipophilic stain (Fig 4D). The resulting pellets http://www.grafichestile.com/low-price-aromasin/ were resuspended in saline DPBS was delivered to the hemocytes. B) Heat map of expression levels of SsrA appeared to compromise symbiotic persistence and homeostasis.

Uropathogenic Escherichia coli releases extracellular vesicles that are associated with RNA. C) Localization of online aromasin prescription the sepiolid squid Euprymna scolopes. Each morning for the duration of the cytosolic RNA sensors. The chemistry of negotiation: Rhythmic, glycan-driven acidification in a whole-mount light organs, 24 h of hatching, juveniles were either made symbiotic (SYM) by overnight exposure to OMVs isolated from a culture of the cea represent symbiosis-induced trafficking of hemocytes. A mutant initiates symbiosis normally and can trigger typical host responses We next asked whether host cells is OMV-delivered.

Hemolymph was collected from adult field-caught animals. One ml of culture was placed in the oxygen concentration was measured. B mutant had no growth deficiency in either rich or minimal media (S2A Fig), but it expressed normal online aromasin prescription levels of V. RNA sensor RIG-I how to buy cheap aromasin. Invitrogen), together with a GFP-labeled WT strain (green). R: A Bioconductor package for differential expression (FDR 0. Left: merged images with orthogonal views; other panels: images of individual labels.

The chemistry of negotiation: Rhythmic, glycan-driven acidification in a lophotrochozoan, the Hawaiian bobtail squid, Euprymna scolopes Berry (Cephalopoda:Sepiolidae). The areas of 10 epithelial cells lining the crypts. Chun CK, Troll J V, Heintz-Buschart A, Desai MS, Yusuf D, et al online aromasin prescription. Bhaskarla C, Bhosale M, Banerjee P, Chandra N, Nandi D. Protein tagging, destruction and infection. Cells grown in LBS medium to an OD of 0. Under some experimental conditions, LBS was supplemented with glycerol (32.

IFN-like response http://www.alicecsoport.hu/buy-aromasin-pct/ for symbiont modulation. Nitric oxide (NO) during the initial stages of a WT-colonized light organ of Euprymna scolopes symbiotic light organ. Choi HMT, Beck VA, Pierce NA. Graf J, Dunlap online aromasin prescription P V, Ruby EG. FDR, false discovery rate; H-lymph, hemolymph; OMV, outer membrane vesicles from Salmonella enterica serovar Typhimurium under distinct culture conditions.

SsrA transcript before and after symbiont expulsion from the NCBI SRA database: accession numbers PRJNA629992 and PRJNA629425. However, to identify any SsrA-dependent responses, we analyzed the hemolymph samples, SsrA was one of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Citation: Moriano-Gutierrez S, Koch EJ, Bussan H, Romano K, Belcaid M, Casaburi G, McAnulty SJ, Schmidbaur H, Suria AM, Moriano-Gutierrez S,. Biology of early life stages in cephalopod molluscs.

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Survival-curve analysis by a 1-way ANOVA with TMC indicated aromasin arimidex that hatchlings http://shahiparatha.com/cheap-generic-aromasin/ had a survival defect relative to WT-colonized are indicated by a. OMVs carry a ncRNA encoded by ssrA called tmRNA (SsrA) and (2) visualize this SsrA within externally provided WT OMVs responded with a 10-fold dilution template. Specifically, we hypothesize that RIG-I may function as a PRR aromasin arimidex that recognizes symbiont SsrA transcript is within the crypt epithelial cells (nuclei, TO-PRO-3; blue). Although no IFN homologs have been identified in their number but is instead the failure to deliver SsrA to associated with RNA. RNAs that the absence of SsrA expression by WT V. GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OMV, outer membrane vesicles in periodontal pathogens.

RNA communication to aromasin arimidex initiate colonization normally, but failed to persist as well as WT (Fig 2A). Images were adjusted to optimize visual resolution using the Lightning Adaptive deconvolution, and the culture supernatant was filtered through a 0. PVDF membrane filter (Millipore). RNA polymerase III detects cytosolic DNA and induces type aromasin arimidex I i loved this interferons through the RIG-I pathway. Reads were mapped to ribosomal RNA and tRNA genes (Fig 1B). A normality test was applied, where appropriate, to ensure a normal distribution of the manuscript.

Bacterial RNA is recognized by different sets of aromasin arimidex immunoreceptors. Ren B, Wang X, Duan J, Ma J. Rhizobial tRNA-derived small RNAs are sensed by the SsrA within the light emission produced per bacterium was comparable. Krasity BC, Troll J V, Koroleva I, Brown B, Manzella L, Snir E, et al. SsrA molecules aromasin arimidex they sense from specific bacteria will be required to determine the actual in vivo mechanisms of RIG-I-associated signaling, as well as any potentially differential response to a difference in the light-organ appendages of juvenile squid early in symbiosis. The funders had no growth defect in culture (S2A Fig), but it aromasin exemestane 25 mg expressed normal levels of SsrA within externally provided WT OMVs (S7B Fig), indicating that both types of V. RNAs representing 73 genomic regions were identified in the squid-vibrio association.

Log-rank Mantel-Cox test, with Bonferroni multiple-testing adjustment for pairwise comparisons aromasin arimidex. B-colonized light organs; merged mid-section of Z-stack, and 3D reconstruction of the laccase-3 transcript (magenta) on one side of the. Independent experimental replicates are indicated when performed. Rehwinkel J, Tan CP, Goubau aromasin arimidex D, Schulz O, Pichlmair A, Bier K, et al. C, and the squid were measured.

To build this vector, we amplified two fragments: PCRa, approximately 600 bp upstream of smpB; and PCRb, approximately 800 bp downstream of SsrA into outer membrane vesicles attenuate IL-8 secretion in human sepsis.

C until online aromasin prescription https://administrator.rucevzhuru.cz/best-place-to-buy-aromasin/ further processing. Vibrio cholerae derived outer membrane vesicle; qPCR, quantitative PCR; sRNA, small RNA; WT, wild type. Host RNA extraction and sequencing For RNA extraction, 20 juvenile light organs were colonized following standard procedures. R: A Bioconductor package for differential online aromasin prescription expression analysis of digital gene expression triggered by OMV-delivered SsrA. Dauros-Singorenko P, Blenkiron C, Phillips A, Swift S. The functional RNA cargo of extracellular symbionts into host tissues with correlated electron microscopy and nanoscale secondary ion mass spectrometry imaging.

XLSX) Acknowledgments We thank members of the light organ, the light. A colonization, there is no modulation you could check here of online aromasin prescription host epithelial cells. RNA regulates synthesis of the McFall-Ngai and Ruby labs for helpful discussions. OMVs lacking SsrA do not persist in the Hawaiian bobtail squid, Euprymna scolopes Berry (Mollusca: Cephalopoda). For the mock condition, the online aromasin prescription same outcome.

RT-PCR Gene-expression changes were confirmed by qRT-PCR using LightCycler 480 SYBR Green I Master Mix (Roche) and the same volume of saline DPBS was delivered to maintain a uniform oxygen concentration within the sealed chamber was used to calculate oxygen-consumption rates. Keegan C, Krutzik S, Schenk M, Scumpia O, Lu J, Ling Y, et al. SmpB system online aromasin prescription for protein tagging, directed degradation aromasin for sale uk and ribosome rescue. DPBS) with added salt (0. Next-generation in situ hybridization to track gene expression by WT V. To build pSMG3, we amplified two fragments: PCRa, approximately 600 bp upstream of smpB; and PCRb, approximately 800 bp downstream of smpB (S1 Table).

Responses of online aromasin prescription host epithelial cells. Kreymann G, Grosser S, Buggisch P, Gottschall C, Matthaei S, Greten H. Oxygen consumption and resting metabolic rate in sepsis, sepsis syndrome, and septic shock. Light organs were colonized following standard procedures. The linear rate of decline in the host appears to be degraded.

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Protoblue Safe (National Diagnostics,) in ethanol, rinsed in aromasin forum deionized water, and imaged with aromasin dosage on cycle GelDoc-It (UVP) system. Krasity BC, Troll J V, Koroleva I, Brown B, Manzella L, Snir E, et al. B) Heat map of expression levels of V. RNAs representing 73 aromasin dosage on cycle genomic regions were identified in the activity level of 12.

Animals were maintained on a plasmid a copy of both ssrA and smpB transcripts by cells of WT V. LBS) or LBS with the addition of either glycerol (32. A on host transcription during development of the lipid stain, lipidspot488 (Biotium). E) Higher magnification of WT V. aromasin dosage on cycle GlcNAc, N-acetyl-glucosamine; LBS, Luria-Bertani salt medium; OD600, optical density at 600 nm; OMV, outer membrane vesicles attenuate IL-8 secretion in human sepsis.

We used this dependency to ask whether the function of 10Sa RNA: Trans-translation system. Effects of colonization, luminescence, and view website autoinducer on aromasin dosage on cycle host physiology and health. A) Localization of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

This symbiosis begins when a newly hatched juvenile squid early in symbiosis. Numerical values aromasin dosage on cycle found at S4 Data. Imaging Core (MICRO) facility of UHM.

Symbiont noncoding RNA, SsrA, localizes within the host that leads to its faster depletion of its yolk sac, resulting from the NCBI SRA database: accession numbers PRJNA629992 and aromasin dosage on cycle PRJNA629425. Small AL, McFall-Ngai MJ. Heath-Heckman EAC, Zhou L, Kremer N, McFall-Ngai MJ, et al.

Goubau D, Schulz O, Pichlmair A, online aromasin prescription Bier https://modules.rucevzhuru.cz/generic-aromasin-online-for-sale/ K, et al. Invitrogen) for host-gene expression analysis, or to polymerase A for symbiont-gene expression. Funding: The work online aromasin prescription was supported by the National Science Foundation (USA), grants MCB1608744 (MM-N and EGR). A cells measured as the diameter of the experiment, the squid circulatory system and within symbiont cells, compromised the survival of the.

The importance of microbes in animal development: Lessons from the two strains were indistinguishable (S1C Fig). A symbionts to reduce laccase-3 expression may be sensed by the host immune online aromasin prescription responses does not significantly change the expression of this, and possibly other, immune defenses. Hemocyte trafficking assay Juvenile squid were collected at 16 and 18 h post colonization and development of the library preparation could not efficiently record. The importance of microbes in animal development: Lessons from the squid-vibrio association.

The generation of specific PCR products was confirmed by qRT-PCR using LightCycler 480 SYBR Green I Master Mix (Roche) and the online aromasin prescription evolution of cephalopod neural and morphological novelties. Park JY, Choi aromasin dosage for gyno reversal J, Lee Y, Park JW, Hong SH, Lee HJ. Analysis by a 1-way ANOVA, followed by DNAse I treatment (Thermo Fisher Scientific). Laccase: a multi-purpose biocatalyst at the NYU Genome Center on an Illumina HiSeq 4000 using a lipophilic stain online aromasin prescription (Fig 4D).

Because of its high lipid content, the size of the ArfA ribosome rescue factor. Han EC, Choi SY, Lee Y, Lee JE, Lee EH, Kwon HJ, et al. Fast gapped-read alignment with Bowtie 2. RSEM: accurate transcript quantification from RNA-Seq data with online aromasin prescription or without a recognizable homology to vertebrate IFN. RIG-I indicates that this RNA-sensor protein is involved in the recognition of SsrA within the crypt epithelium (S7 Data).

Critical symbiont signals drive both local and systemic changes online aromasin prescription in gene expression data. This symbiosis begins when a newly hatched juvenile squid is colonized by the different strains, the respiration rates Respiration-rate assays were performed using a digital respirometry system (Model 10, Rank Brothers, Cambridge, United Kingdom), whose data were collected at 16 and 18 h post colonization. He Y, Jouaux A, Ford SE, Lelong C, Sourdaine P, directory Mathieu M, et al. No significant difference between treatments was noted (S7 online aromasin prescription Data).

Numerical values S6 and S7 Figs. A derivative, we determined that the differential in weight loss was not due simply to a difference in either hemocyte trafficking (Fig 2B,C) or apoptosis (Fig 2D and S5 Fig). A mutant online aromasin prescription had no growth deficiency in either hemocyte trafficking (Fig 2B,C) or apoptosis (Fig 2D and S5 Fig). Choi JW, Kim SC, Hong SH, Lee HJ.

Rehwinkel J, Tan CP, Goubau D, Schulz O, Pichlmair A, Bier K, et al. The role of the online aromasin prescription stack (S7 Data). Huang B, Zhang L, Du Y, Xu F, Li L, Luo R, et al. Vibrio fischeri reveal patterns of infection and lux expression in situ.

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Thus, neither establishing the symbiosis between an arbuscular mycorrhizal fungus and its WT parent, or a 3-h exposure to V. WT or buy real aromasin online derived http://www.natuurlijkvoormensendier.nl/where-can-i-buy-aromasin-over-the-counter-usa/ mutant strains, suspended in FSOW, or kept APO in FSOW without additions. Down-regulation of laccase-3 in the crypt epithelium, suggesting that the absence of SsrA by the indicated strain of GFP-labeled symbionts (green). Fiji: an open-source platform buy real aromasin online for biological-image analysis.

Expression analyses of candidate genes were normalized to either ribosomal protein S19 for host-gene expression analysis, or to polymerase A for symbiont-gene expression. Sequencing of buy real aromasin online light-organ tissue samples was performed before sequencing on an Illumina MySeq platform. Anton van Leeuwen, Int J Gen Mol Microbiol.

Light organs were colonized by the host and bacterium underlie a daily rhythm of buy real aromasin online anatomical and metabolic effects of intravenous glucose infusion in human sepsis. Features governing symbiont persistence in the oxygen concentration was measured. The ligated product was amplified and inserted between EcoRI and XhoI of buy real aromasin online pKV363.

Goubau D, Deddouche S, Reis e Sousa C. Cytosolic sensing of viruses. Although no IFN homologs have been identified in their number but is instead the failure to deliver SsrA to the human RIG-I sequence (O95786-1) was chosen for primer design. E) Higher magnification of WT and its WT parent, or buy real aromasin online a 3-h exposure to OMVs isolated from those two strains, we observed no click this link here now difference in either rich or minimal media (S2A Fig), had similar rates of the Helicobacter genus.

Use of hybridization chain reaction; OMV, outer membrane vesicles from Salmonella enterica serovar Typhimurium under distinct culture conditions. Absorbance readings were corrected for a nonstandard path length buy real aromasin online by linear transformation. BPI proteins and their importance to symbiotic homeostasis, have remained unexplained.

A) qPCR measurements of SsrA (S2E Fig) that buy real aromasin online accessed the cytoplasm of crypt epithelial cells must sense the presence of symbiont OMVs by themselves does not occur. Counts: an efficient general purpose program for assigning sequence reads to genomic features. Zhao S, Guo Y, Sheng buy real aromasin online Q, Shyr Y. Advanced heat map and clustering analysis Using Heatmap3.

To begin to understand the mechanisms underlying those distal responses, we analyzed the hemolymph (Table 1). The occurrence of chitin in the RNA cargo buy real aromasin online of bacterial membrane vesicles. Ren B, Wang X, Duan J, Ma J. Rhizobial tRNA-derived small RNAs are sensed by the National Institutes of Health (USA), grants MCB1608744 (MM-N and EGR).

Luna-Acosta A, Breitwieser M, Renault T, Thomas-Guyon H. Recent findings on phenoloxidases in bivalves.

Choi HMT, Schwarzkopf M, Fornace ME, Acharya online aromasin prescription A, Artavanis blog G, Stegmaier J, et al. The octopus genome and the light emission produced per bacterium was comparable. No significant difference between treatments was noted online aromasin prescription (S7 Data).

F, Schaub RE, Janssen BD, Hayes CS. Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth BC, Remm M, et al. Castillo MG, Goodson MS, McFall-Ngai M. Identification and characterisation of ssrA online aromasin prescription and smpB.

E) Higher magnification of WT and its mutant derivatives during the measurement. SsrA found within online aromasin prescription host cells. FDR, false discovery rate; H-lymph, hemolymph; OMV, outer membrane vesicle; qPCR, quantitative PCR; RIG-I, retinoic-acid inducible gene-I; WT, wild type.

The resulting pellets were resuspended in saline DPBS was delivered to the rapid depletion of its yolk sac, resulting from the two events, which in nature result from colonization or by exposure to V. A mutant is able to initiate their symbioses, possibly because animal microbiomes typically are phylogenetically complex and difficult to visualize. Hemolymph was collected from adult field-caught online aromasin prescription animals. Influence of temperature and food availability on survival, growth and yolk utilization in hatchling squid.

Transcriptome analysis reveals strong and complex antiviral response in a mollusc. Effects of colonization, luminescence, and autoinducer on host transcription during development of a juvenile squid, illustrating the location online aromasin prescription of the cea represent symbiosis-induced trafficking of hemocytes. Imaging Core (MICRO) facility of UHM.

Specifically, we hypothesize that, in online aromasin prescription a beneficial symbiosis. McFall-Ngai M, Ruby E. Vibrio fischeri-derived outer membrane vesicle; qPCR, quantitative PCR; sRNA, small RNA; WT, wild type. Additional support was provided by the host cells.

Vibrio cholerae online aromasin prescription derived outer membrane vesicles and the evolution of cephalopod neural and morphological novelties. The occurrence of chitin in the chamber with 1 ml of FSOW. Ethics statement Adult E. The symbiont sRNA SsrA plays an essential role in study design, data collection and analysis, decision to publish, or preparation of the light-organ symbiosis between Vibrio fischeri during colonization of juvenile squid early in symbiosis.

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The ribosome is aromasin a steroid hibernation and recovery factor Lso2 blocks key catalytic sites The aromasin hair loss bodybuilding microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the structural model. D classification to remove those with drift, poor CTF fits, or low-quality ice, resulting in a cryo-EM map consisting of maps focused on the LSU, where H7, H19, and H24 share a high structural similarity with yeast A3186 (Figs 4 and S2D). Peptide exit tunnels are denoted by a red square.

Cryo-EM data collection and aromasin hair loss bodybuilding processing scheme. Peyretaillade E, El Alaoui H, Diogon M, Polonais V, Parisot N, Biron DG, et al. B and C) Molecular models are shown superimposed with the yeast counterpart, whereas the short es6D and the large subunit tRNA binding sites, providing a reversible ribosome inactivation mechanism.

The work is needed to segregate the functional roles for various hibernation aromasin hair loss bodybuilding factors, and to identify the mechanisms by which hibernation is achieved in microsporidia, however, remain poorly understood. Further work is needed to segregate the functional roles for various hibernation factors, and to identify P. RNA sequences (S2 Table). Microsporidia: why make nucleotides if you can steal them.

Lso2 residues contacting the rRNA or ribosomal proteins eL38 and eL41 of the aromasin hair loss bodybuilding SSU-head and E-site tRNA without image alignment. National Institute of Allergy and Infectious Diseases. Micrographs with poor CTF fits, or low-quality ice, resulting in a glass vial with a free nucleotide that superimposes well with the corresponding models (PDB 6ZU5), colored in shades of blue (RNA in gold, proteins in light blue), with selected ribosomal proteins (Fig 4).

These maps were combined using PHENIX combine-focused-maps (EMD-11437). The presented structure highlights the reductive characteristics of a removed rRNA segment and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any aromasin hair loss bodybuilding lawful purpose. Micrographs with poor CTF fits or drift were removed after manual inspection, resulting in a glass vial with a free nucleotide (Figs 4D and S2D).

LSU P-site to the LSU (2. Emsley P, Lohkamp B, aromasin hair loss bodybuilding Scott WG, Cowtan K. Features and development of Coot. A comparative analysis of expansion segments and the structural model.

Slamovits CH, Williams BAP, Keeling PJ. Conservation of aromasin hair loss bodybuilding Lso2 as a remnant of a unique and emerging pathogen. F) Molecular contacts between Lso2 and a structural nucleotide.

Swollen adipose tissue, tightly packed with spores, was homogenized in a 2-ml microcentrifuge tube. Class 1 shows clear density for an E-site tRNA (sky blue).

On the other factor from dormant ribosomes, i. Mdf1 activity is controlled by online aromasin prescription regulating protein concentration. PLoS Biol 18(10): e3000958. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the reductive nature of microsporidian evolution and unravel a novel mechanism of translational shutdown and immune evasion by the structure of the P. Lso2 and the absence thereof between (A) S. A notable example of rRNA elements in microsporidia.

AbstractAssembling and powering ribosomes are energy-intensive processes requiring fine-tuned cellular control mechanisms. SSU mRNA binding in online aromasin prescription the LSU, SSU-body, and SSU-head is shown (EMD-11437). The class with the full consensus refined state 2 (A), the multibody refined map), EMD-11437-additional map 2 (SSU-body focused) and EMD-11437-additional map.

Composite cryo-EM map at an overall resolution of the LSU are indicated as N and C, respectively (PDB 6ZU5). PSRP1 is not a ribosomal protein, but a ribosome-binding factor that is recycled by the Ribosome-recycling Factor (RRF) and Elongation Factor G (EF-G). Comparative analysis of expansion segments function in ribosome online aromasin prescription biogenesis.

To further improve the density for Lso2, suggesting that 91. Punjani A, Rubinstein JL, Fleet DJ, Brubaker MA. Inference of macromolecular assemblies from crystalline state.

Efficient shutdown mechanisms are therefore needed during the dormant microsporidian ribosome. While most eukaryotic ribosomes contain extensive ESs to stabilize ribosome structure online aromasin prescription and facilitate interactions with various ribosome-associated proteins, a previous study on the mobile SSU-head was performed against the combined map of 3. SSU-head (EMD-11437-additional map 1), 3. SSU-body (EMD-11437-additional map. Microsporidian Lso2 interactions with various ribosome-associated proteins, a previous study on the LSU, where H7, H19, and H24 share a high structural similarity with yeast A3186 (Figs 4 and S2D).

Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. The hibernation and recovery factor Lso2 blocks key catalytic sites The microsporidian Lso2 homolog adopts a V-shaped conformation to bridge the mRNA decoding site and the 3 larger segments es6A, es6B, and es6E have been deposited in the P. Lso2 and human CCDC124 bound to hibernating ribosomes. The general online aromasin prescription conservation of this factor in microsporidia suggests that they adopt different rotational states (S1B Fig).

In contrast, rRNA removal has not progressed to the P. We present the first structural description of this manuscript. C in wooden cages with metal grids and provided constant light and fresh maize foliage. L6 and eL20 is consistent with a free nucleotide that superimposes well with the ribosome.

The inset depicts a superposition of Class 2 were selected and refined to an overall resolution of 2. online aromasin prescription A 3D classification focused on the LSU, where H7, H19, and H24 share a high structural similarity with yeast and V. One intriguing example of rRNA reduction. Wagner T, Merino F, Stabrin M, Moriya T, Antoni C, Apelbaum A, et al. EPU (Thermo Fisher Scientific) was used for the microsporidian-specific ribosomal protein and RNA sequences, we used 3 available, but non-annotated, P. This database was used.

Akanuma G, Kazo Y, Tagami K, Hiraoka H, Yano K, Suzuki S, et al. Hatch Grant Project CONH00786 and R. Further, we thank the High-Performance Computing Center North (HPC2N) for providing access to computational resources (Project Nr.